Skills and methods

Skills

Immunochemistry, Immunology, Biochemistry and physical chemistry of proteins, Proteomics, Cell biology and in vitro models, Digestion, Translational research approaches: preclinical models and clinical studies, Functional exploration of organs.

Methods

•  Allergen purification (sequential extraction, chromatography)
•  Production of polyclonal and monoclonal antibodies - collection of anti-allergen antibodies (wheat, pea, rapeseed, egg, milk, etc.) - serum library
•  Detection and assay of immunoglobulins and cytokines by ELISA method
•  Characterisation and assay of allergens using proteomics (electrophoresis, western blot, dot blot, mass spectrometry)
•  Identification of epitopes using synthetic peptides (pepscan)
•  Digestive hydrolysis, in vitro study of allergen resistance
•  Models for studying allergy mechanisms
  •  in vitro: epithelial cells (Caco 2, etc.), basophils (humanised RBL)
  •  ex vivo: intestinal biopsies, primary cells (spleen, mesenteric lymph nodes, etc.)
  •  in vivo: preclinical mouse models of allergy (food, asthma, atopic gait)
•  Analysis of cell populations and receptor labelling using flow cytometry
•  Clinical study with Nantes University Hospital