Fruit skin polymers characterized by Maldi imaging

Fruit skin polymers characterized by Maldi imaging

Maldi Imaging: A tool to better characterize polymers in fruit skin

The BIA unit in Nantes has developed a method for analyzing cutin, including an in situ depolymerization step followed by mass spectrometric imaging. This makes it possible not only to analyze the nature of the monomers but also to access their spatial distribution (with a resolution of the order of 50 μm). This imaging technique allows for the chemical and spatial characterization of cutin polymers (figure below).

Maldi-cutine

Difference in composition of cutin monomer revealed by MALDI imaging in suberized areas.
(a) MALDI image; (b) skin photo.

See also

Associated publication: Velickovic, D., Herdier, H., Philippe, G., Marion, D., Rogniaux, H., and Bakan, B. (2014). Matrix-assisted laser desorption/ionization mass spectrometry imaging: a powerful tool for probing the molecular topology of plant cutin polymer. Plant J 80, 926-935.